Protein_Domain

Part:BBa_K2355026

Designed by: Cathrine Agnete Larsen   Group: iGEM17_DTU-Denmark   (2017-10-26)


Cleavable peptide sequence from Human Calcium/calmodulin-dependent protein kinase type IV

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis arietans. The specific peptide sequence of the linker is PAPDAPLK.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Results of experiments

This peptide is one out of three that were specifically cleaved only by Bitis arietans.

In the graf below you are able to see the cleavage by all three venoms.

Substrate screening data for K23
Figure 1: Substrates with sequences PAPDAPLK (well K23) incubated with the three different venoms.

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